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The prognostic model's predictive efficacy was evaluated using the area under the subject operating characteristic curve (AUC), sensitivity, and specificity

Plasma trimethylamine N-oxide and its precursors: population epidemiology, parentchild concordance, and associations with reported dietary intake in 11- to 12-year-old children and their parents

SuperScript IV reverse transcriptase (Invitrogen) was used for cDNA synthesis and for transcript sequencing reactions, which used a 2:1 ratio of dNTPs to ddNTPs

Use multiple timepoints to capture progression, standardize assays and scoring criteria, and prespecify primary and secondary endpoints to guide analysis and reduce selective reporting