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coli strain XL1-Blue and purified by affinity chromatography using an elution buffer containing 200 mM imidazole, 300 mM NaCl and 50 mM sodium phosphate, pH 8.0 (refs 11, 12, 18, 33, 53)

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Allow vial to reach room temperature before preparation Draw 2ml bacteriostatic water using a sterile syringe Inject slowly down the inside wall of the vial Avoid foaming Gently swirl until fully dissolved Do not shake vigorously Label with the reconstitution date Refrigerate at 2C8C Protect from direct light exposure Protocol Overview Goal: Support Testicular Function, Fertility & Hormonal Balance Research Route: Subcutaneous Schedule: 3 Times Weekly Dose Range: 500 IU 2,000 IU Per Injection Reconstitution: 2ml BAC Water Final Concentration: 2,500 IU/ml Storage: Refrigerated After Reconstitution Reference Parameters (Standard-Dose protocol) For laboratory research only