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Polyamine synthase activity: 0.3 g fresh leaf sample was ground into a homogenate in potassium phosphate buffer (100 mm, pH 8.0) containing 0.1 mm phenylmethylsulfonyl fluoride, 1 mM pyridoxal phosphate (PLP), 5 mm EDTA, 25 mm ascorbic acid, and 0.1% polyvinylpyrrolidone

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