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After incubation for 4 h at 37 C in 5% CO 2 humidified atmosphere, colony forming units were determined on Barratts minimal medium as described earlier

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Furthermore, the first three eigenvectors were collected from each MD simulation trajectory and plotted to demonstrate the residual displacement in the different conformations of the protein structure, where a gradient color change (from blue to white to red) specifies that there are regular leaps among the various conformation of protein structure throughout the trajectory (Fig