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K.BraatenB

Astrocyte-specific expression of a soluble form of the murine complement control protein Crry confers demyelination protection in the cuprizone model

Discussion We show here that Rev1 is remarkably well-adapted for the error-free bypass of 10 S (+)- trans -BP- N 2 -dG, 10 R (+)- cis -BP- N 2 -dG, and 10 S ()- cis -BP- N 2 -dG stereoisomeric adducts

For PCR analysis, 2 L of the lysate was used as a template with the following primers: F1: 5-GCCAGCTACTGCATATATTGAGACC-3 R1: 5-GGATATAGAGAAGCCCTAGAACAGGG-3 R2: 5-CATGTCGTGTTGGTCAAATAAGCAC-3 PCR amplification using primers F1/R1 generated a 278 bp product for wild-type (WT) alleles and a 382 bp product for floxed alleles