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After centrifugation, 5.5-dithiobis (2-nitrobenzoic acid) (DTNB) solution was added into the supernatant and the absorbance of the compound was recorded by using UV-vis spectrophotometer (Perkin Elmer, Waltham, MA)

Secondly, 2,4-D showing pro-oxidant activity in living cells can interfere with the proper functioning of enzymes responsible for optimal regulation of GSH biosynthesis, GSH/GSSG ratio and cellular redox homeostasis, which may lead to a significant weakening of the cells antioxidant potential and a deepening of the toxic and genotoxic effects of 2,4-D 69,72