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Recent work has also indicated that BSO can potentiate the toxicity of TMZ-induced bystander effect on glioblastoma cells 36 and a GSH transferase inhibitor was shown to potentiate the cytotoxic effect of TMZ in melanoma cells

The supernatants of the cell lysates were isolated at 12,500 g for 30 min at 4 C and were incubated with anti-Flag magnetic beads overnight at 4 C, followed by extensive washing with NP-40 Lysis Buffer