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doi: 10.1038/sj.bjp.0706912 123 BrhlHCihakJPlachJKunz-SchughartLNiedermeierMDenzelAet al

Biomedicines 11 , 2818 (2023)

Huai et al

Mixtures of the designated purified proteins were incubated with glutathione Sepharose beads or Ni-NTA beads in pull-down buffer (20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1 mM EDTA, 1 mM DTT, 1 protease inhibitor) at 4 C for 2 h