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TMAO may contribute to the development of MAFLD through the following mechanisms (Figure 1)

Starting from RNA extracted with the Abbott m 2000 sp system, retrotranscription reactions were performed in a 30 l volume containing 7.5 l eluted RNA, 0.25 mM dNTPs, 150 U Moloney murine leukaemia virus reverse transcriptase and 6 l 5 RT buffer (Promega), 30 U RNasin RNase inhibitor (Promega), and 0.75 g random hexadeoxynucleotides (Roche) in order to retrotranscribe the whole HCV genome preventing any bias during the reaction

However, one question that commonly arises is whether the results are permanent

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