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[DOI] [PubMed] [Google Scholar] 13.Kaur G., Tak Y., Asthir B

Cells were lysed in a cell lysis buffer (50 mM TrisHCl, pH 6.8, containing 10% glycerol, 2% SDS, and 1% proteinase inhibitor cocktail)

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To understand the mechanism by which rhGH could stimulate the early onset of puberty in males, changes in hypothalamic kisspeptin, GnRH and IGF1 levels, pituitary and circulating LH, spermatogenesis, testicular steroidogenesis, and IGF1 in the circulation, liver, and testes were examined in immature rats following prepubertal rhGH treatment