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Rumen microbes rely on something called the intrinsic factor , a glycoprotein that is derived from the degradation of non-protein nitrogen sources (such as urea or ammonia) or other proteins, so that cobalamin can be absorbed

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Procedure: Cleanse meticulously by suitable means, followed preferably by heating at 250 for 2 hours, hard-glass test tubes, about 20 mm x 150 mm in size, and other necessary glassware because of the high sensitivity of the test organism to minute amounts of vitamin B 12 activity and to traces of many cleansing agents

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