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Root-associated defence mechanisms include Fe 3+ plaque formation, enhanced aerenchyma development, and apoplastic Fe sequestration 20

NADH oxidation (FSP1 activity) assay To measure NADH consumption, 2.5 pmol of immunopurified FSP1GFP isolated from indicated cell lines or recombinant WT or D285N mutant FSP1 at the indicated concentrations were combined with 500 M NADH and 400 M CoQ1 in a total volume of 200 l in 50 mM sodium phosphate pH 7.4

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