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Krischer et al

However, direct clinical evidence demonstrating this causal sequence in patients remains limited and warrants further validation using in vivo or ex vivo barrierfunction assays

This reduced systemic utilization indicates the overall effect of intestinal breakdown, deficient absorption, and rapid metabolic transformation

Importantly, however, particle design governs risk: surface-displayed antigens or certain chemistries can raise immunogenicity and, in rare cases, provoke severe hypersensitivity (including anaphylaxis), whereas encapsulation strategies tend to blunt immediate antigen recognition and are therefore often safer in vivo (206, 208)