INOV2a, ACAGTTGTAAGGGATCTTGTGGCTGTCAGGATGGCAGAGGAGCAGGAGTTCACCCAGC jCG 105757-01 TCTGCAAGTTGCCTGCACAGCCCTCACACCCACACTGCGTGAACAACACCTACCGCAG DNA Sequence CGCACAGCACTCCCAGGCTCTGCTCCGAGGGCTGCTGGCTCTCCGGGACAGCGGAATC CTCTTCGATGTTGTGCTGGTGGTGGAGGGCAGACACATCGAGGCCCATCGCATCCTGC TGGCTGCGTCCTGCGATTACTTCAGGAGAGGAATGTTTGCTGGGGGATTGAAGGAGAT GGAACAGGAAGAGGTCCTGATCCACGGTGTGTCCTACAATGCTATGTGCCAAATCCTA CATTTCATATACACCTCCGAGCTGGAGCTCAGCCTGAGCAATGTACAAGAGACACTGG TGGCTGCCTGCCAGCTGCAGATCCCAGAAATTATCCATTTCTGCTGTGATTTCCTCAT GTCCTGGGTGGACGAAGAGAACATTCTCGATGTCTACCGGCTGGCAGAGCTGTTTGAC TTGAGCCGCCTGACTGAGCAACTGGACACCTATATCCTCAAAAACTTTGTGGCCTTCT CTCGGACTGACAAGTACCGCCAGCTTCCATTGGAGAAGGTCTACTCCCTCCTCAGCAG CAATCGCCTGGAGGTCTCCTGCGAGACCGAGGTATATGAGGGGGCCCTTCTCTACCAT TATAGCCTGGAGCAGGTGCAGGCTGACCAGATCTCGCTGCACGAGCCCCCAAAGCTCC TTGAGACAGTGCGGTTTCCGCTGATGGAAGCTGAGGTCCTGCAGCGGCTGCATGACAA GCTGGACCCCAGCCCTTTGAGGGACACAGTGGCCAGCGCCCTCATGTACCACCGGAAC GAGAGCCTACAGCCCAGCCTGCAGAGCCCGCAAACGGAGCTGCGGTCGGACTTCCAGT GCGTTGTGGGCTTCGGGGGCATTCACTCCACGCCGTCCACTGTCCTCAGCGACCAGGC CAAGTATCTAAACCCCTTACTGGGAGAGTGGAAGCACTTCACTGCCTCCCTGGCCCCC CGCATGTCCAACCAGGGCATCGCGGTGCTCAACAACTTCGTATACTTGATTGGAGGGG ACAACAATGTCCAAGGATTTCGAGCAGAGTCCCGATGCTGGAGGTATGACCCACGGCA CAACCGCTGGTTCCAGATCCAGTCCCTGCAGCAGGAGCACGCCGACCTGTCCGTGTGT GTTGTAGGCAGGTACATCTACGCTGTGGCGGGCCGTGACTACCACAATGACCTGAATG CTGTGGAGCGCTACGACCCTGCCACCAACTCCTGGGCATACGTGGCCCCACTCAAGAG GGAGGTAGTGTATGCCCACGCAGGCGCGACGCTGGAGGGGAAGATGTATATCACCTGC GGCCGCAGAGGGGAGGATTACCTGAAAGAGACACACTGCTACGATCCAGGCAGCAACA CTTGGCACACACTGGCTGATGGGCCTGTGCGGCGCGCCTGGCACGGCATGGCAACCCT CCTCAACAAGCTGTATGTGATCGGGGGCAGCAACAACGATGCCGGATACAGGAGGGAC GTGCACCAGCTCCCAGGTGCCCACGTGCTGCGCTGGCTGGAGGCAGCAAGGGGACGAG TGTGGGATTGCGGTGTGCGAAGGCAACTCCACGTGCTCAGAGAACGAGGTGTGCGTGA^ GGCCTGGCGAGTGCCGCTGCCGCCACGGCTACTTCGGTGCCAACTGCGACACCAGTGT GGCCAGTGCAAGGGGCCAGCAGCCGTGCACGGTGGCCGAGGGCCGCTGCTTGACGTGC GAGCCCGGCTGGAACGGAACCAAGTGCGACCAGCCTTGCGCCACCGGTTTCTATGGCG AGGGCTGCAGCCACCGCTGTCCGCCATGCCGCGACGGGCATGCCTGTAACCATGTCAC CGGCAAGTGTACGCGCTGCAACGCGGGCTGGATCGGCGACCGGTGCGAGACCAAGTGT AGCAATGGCACTTACGGCGAGGACTGCGCCTTCGTGTGCGCCGACTGCGGCAGCGGAC ACTGCGACTTCCAGTCGGGGCGCTGCCTGTGCAGCCCTGGCGTCCACGGGCCCCAGTG AGTGCCCCGGGACCGGGAGGGGGTTGGGGGCTTGTACCTGCCACAGAGGGGGGTCCAG CCGACGAGGTGGCCTCTCCACCCTGAGCTGGGTTATCACCTCAGCCTTGGTCCCTTAC CCCAGCTAGGGAGTGACAGTAGGCTCTTTGGGGGCAGTTTCCTGCCTGGATGTCGGGG jAGC'fCACGTTCAGCGCAGGATCTGGTGACCAGTCCAGCCTGTGTCAGTGGGCTCTTAA GGTGACCCCGAGTTGGTACAGAAGGACCAGGGACCTCCACTTACAGCCAAGGGTCTGG TTCAGCAGCCCCTCTTCCCACCTAGCCGAGTCAGCCCCAGCAGTGGGCGCTGCGGCGC GGCCACCACGGGTCCTATCCCCCAGGCCCCCCCACTAGTGTTGTGCAACATTCGTTTC CAAAACATCCACTACCCAATATGTGCC ORF Start: ATG at 31 ORF Stop: TAA at 1903 SEQ ID NO: 16 624 aa MWat 71369.7 Da lNOV2a, MAEEQEFTQLCKLPAQPSHPHCVNNTYRSAQHSQALLRGLLALRDSGILFDWLWEG JCG105757-01 RHIEAHRILLAASCDYFRRGMFAGGLKEMEQEEVLIHGVSYNA CQILHFIYTSELEL iProtein Sequence SLSNVQETLVAACQLQIPEIIHFCCDFLMS*WVDEENILDVYRLAELFDLSRLTEQLDT YILKNFVAFSRTDKYRQLPLEKVYSLLSSNRLEVSCETEVYEGALLYHYSLEQVQADQ ISLHEPPKLLETVRFPLMEAEVLQRLHDKLDPSPLRDTVASALMYHRNESLQPSLQSP QTELRSDFQCWGFGGIHSTPSTVLSDQAKYLNPLLGEWKHFTASLAPRMSNQGIAVL NNFVYLIGGDNNVQGFRAESRC RYDPRHNR FQIQSLQQEHADLSVCVVGRYIYAVA GRDYHNDLNAVERYDPATNSWAYVAPLKREWYAHAGATLEGKMYITCGRRGEDYLKE THCYDPGSNTWHTLADGPVRRAWHG ATLLNKLYVIGGSNNDAGYRRDVHQLPGAHVL R LEAARGRVDCGVRRQLHVLRERGVREA RVPLPPRLLRCQLRHQCGQCKGPAAVH GGRGPLLDVRARLERNQVRPALRHRFLWRGLQPPLSAMPRRACL Further analysis of the NOV2a protein yielded the following properties shown in Table 2B
Representative examples of polypeptide fragments of the invention, include, for example, fragments comprising, or alternatively consisting of, from about amino acid number 1-20, 21-40, 41-60, 61-80, 81-100, 102-120, 121-140, 141-160, or 161 to the end of the coding region
Hence, the assignment of the glutathione-scaffold site could facilitate the prediction of disulfide substrate preferences of uncharacterized members of the thioredoxin superfamily
[DOI] [PubMed] [Google Scholar] 78.Bose A, Roy Burman D, Sikdar B, Patra P
