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Although several PPI inhibitors with improved selectivity have recently been identified, using combined approaches that brought together protein crystallography, ligand NMR spectroscopy, and computational chemistry, unfortunately, none of them has been found to be able to cross the BBB and many are quite large in size and contain carboxylic acids, features which usually prevent or decrease CNS permeability [299]

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Not surprisingly, the iatrogenic-induced compromised intestinal barrier renders the patients to increased risks for the same range of complications as those observed in hematologic patients, in addition to some distinct end-results

For each assay, 2 g of fresh leaf tissue was flash-frozen with liquid nitrogen, ground into a fine powder, and homogenized in an extraction buffer containing 100 mM Tris-HCl (pH 8.0), 1 mM EDTA, 5 mM dithiothreitol (DTT), 0.02% (v/v) Triton X-100, and 10% (v/v) glycerol to enhance protein stability and solubility