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NEM was used in the GSSG assay to hide the thiol group of GSH

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Stock solutions of 10 mM ferrous ammonium sulfate and 100 mM ascorbate were prepared in 1 M Tris, pH 8.0

For more information, including the amount and type of niacin used in the study and additional information about niacin and niacin supplements, see the B Vitamin Supplements Review