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the lysate was clarified by centrifugation at 15,000 g for 15 minutes, protein concentrations were determined using the BCA total protein assay, and then appropriate volumes of the lysates were diluted with 2 reducing Laemmli buffer and heated for ten minutes at 95 C

The trial was permitted by Erzincan Binali Yldirim University Local Animal Ethics Committee (Meeting date: 26.09.2024, Meeting No: 09)

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For example, depleting TF from serum or using RNA interference (RNAi) to downregulate TfR significantly inhibits ferroptosis in mouse embryonic fibroblasts (Gao et al., 2015)