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Thereafter, the cells were incubated with primary mouse monoclonal antibody to H2AX (Ser-139) diluted 1 : 1000 (Upstate Biotechnology) or primary antibody anti-cisplatin (CP9/19) (Abcam, Cambridge, MA, USA) that was diluted 1 : 5000 for 16 h at 4 C

The results confirmed the presence of a strong electrophilic centre at the tertiary carbon for both FCCP and NMY1009, which reacts with nucleophilic GSH

Whether their heterogeneity and different response levels to CD38 mAb in patients contribute to different outcomes following Daratumumab therapy requires more evidence (Figure 5) [55]