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Cells were induced for 20 h before harvesting by centrifugation at 4000 g for 10 min at 23 C, flash-frozen and stored at 80 C until use

Speake PF, Glazier JD, Ayuk PT-Y, Reade M, Sibley CP, DSouza SW (2003) L-Arginine transport across the basal plasma membrane of the syncytiotrophoblast of the human placenta from normal and preeclamptic pregnancies