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Cells undergoing cuproptosis exhibit unique biochemical, genetic, metabolic, and morphological features that distinguish them from other recognized forms of cell death, such as apoptosis, pyroptosis, necroptosis, and ferroptosis [13, 14] (Table 1)

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The samples were then infiltrated with GUS staining solution (0.5 mg mL 1 5-bromo-4-chloro-3-indolyl--D-glucuronic acid, 0.5 mM potassium ferrocyanide, 0.5 mM potassium ferricyanide, 0.1 % (v/v) Triton X-100, 100 mM phosphate buffer, pH 7.0, and 10 mM EDTA) following Jefferson et al