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The influence of the low fluence fractional laser on the penetration of high molecular weight model drug, a polypeptide, FITC and FITC-labeled dextran (MWs of 4 and 150 kDa), was assayed by CSLM, whose image of skin showed a fluorescence increase in the upper and middle dermis, as well as in hair shafts and hair sheaths, evidencing a transfollicular route for high molecular weight substances [66]