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After incubation for 72 h, the supernatant was removed, and viability was assessed by the PrestoBlue assay (Life Technologies, Carlsbad, CA, USA), according to the manufacturers instructions

A few ingredients can treat and prevent dark spots, including vitamin C, niacinamide, ferulic acid, glutathione, AHAs (like glycolic and lactic acid), retinol and its alternatives, and Japanese rice bran

Hydroxocobalamin is the standard UK preparation, administered intramuscularly for conditions like pernicious anaemia and gastrointestinal malabsorption B12 acts as a cofactor in DNA synthesis, red blood cell formation, and nervous system function, supporting energy metabolism pathways Common side effects include injection site reactions, mild gastrointestinal upset, and temporary urine discolouration, with serious reactions being rare NHS access requires GP assessment with blood tests

doi: 10.1016/j.biochi.2016.10.019