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Antioxidant markers, including superoxide dismutase (SOD), glutathione peroxidase (GPx), and Glutathione (GSH), were assessed using the Superoxide Dismutase Activity Assay Kit (ab65354, Abcam, Shanghai, China), Glutathione Peroxidase Assay Kit (ab102530, Abcam, Shanghai, China), and Glutathione Assay Kit (ab65322, Abcam, Shanghai, China), respectively

Assuming an increased ROS-level and a decreased level of glutathione, especially the reduced form, as an important antioxidant in Wobbler motor neurons, it can be concluded that NAC, as a precursor of glutathione, can replenish the GSH content and thus reduce oxidative stress in Wobbler motor neurons resulting in less oxidative DNA damage