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The gut microbiota breaks down choline, l-carnitine, and phosphatidylcholine from the diet to form trimethylamine (TMA), which is subsequently taken into the bloodstream and converted into TMAO by flavin-containing monooxygenase 3 (FMO3) in the liver (Chen et al., 2019)

After pharmacological intervention in 96-well cell culture plates for 24, 48 or 72 h, the culture medium was removed and replaced with 0.1 mg MTT reagent (Beyotime, ST1537) per well dissociated in 100 L basal DMEM (Procell, PM150210)

At first it was established that glucose was the major source of energy for the placenta-fetus unit, but more recent studies demonstrated that FAO play an important role for placental and fetus growth (Shekhawat et al., 2004)

Liepinsh, Risks and benefits of carnitine supplementation in diabetes, Exp Clin Endocrinol Diabetes, Fev 2015, 123(2):95-100