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448 On the basis of a fluorescent probe derived from galactose, a fluorescence platform has been developed for easy longitudinal monitoring of enzymatic activities in biofluids

GSSG generated in these processes can be either recycled to GSH by a NADPH-dependent reaction, catalyzed by GR 3 , or pumped out of the cell by ATP-dependent processes, with multidrug resistance-associated protein-1 (MRP-1) being the major pathway for GSSG efflux 33

Given all these in silico inputs, it was decided to choose a tag such as GST (pGEX-4T-1 vector) which could in principle solubilize the polypeptides and refrain it m forming IBs when overexpressed in a host such as E