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In contrast to drug-sensitive A2780 cells, however, although puromycin-resistant colonies were initially formed in A2780DPP cells in multiple replicate experiments, the colonies formed were relatively small (1020 cells per colony) and were viable for only 48 h (Figure 3A)

Second, excess intracellular iron is sequestered by cytosolic ferritin, and can be dynamically released upon iron depletion via ferritinophagy 139,140