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For Bisulfite Sequencing PCR analysis, the means of methylation percentages of each CG locus of MOD, SEV and CTRL groups at endpoints A and B were compared using two-tailed Wilcoxon test and only those that presented a statistically significant difference were considered

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Research published in biochemical journals has demonstrated that NNMT inhibition can lead to: Increased cellular NAD+ levels Enhanced mitochondrial function Improved metabolic flexibility Potential changes in fat metabolism Altered gene expression related to energy utilization The 5-amino-1-mq compound has shown promise in preclinical models, though human research remains limited