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Serum folate reflects short-term changes in diet and may be altered by recent meals, so fasting for about six hours beforehand is required, and repeated testing is needed to identify true deficiency

There is a similar beneficial effect in the rats with the Pringle maneuver, ischemia, reperfusion, and suprahepatic inferior caval vein occlusion (BuddChiari syndrome) (Gojkovic et al., 2020