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For example, K

Divide the dose 60/40 or 50/50 between morning (fasted) and evening (at least 2 hours after eating)

Tyrosine L-Tyrosine is a crucial amino acid involved in synthesizing catecholamines, including dopamine, norepinephrine, and epinephrine, which play significant roles in the stress response of fish [71]

In general such protocol consists of three major steps: cell lysis and solubilisation of the GST-fusion protein (see cartoon below), recovery of the fusion from the lysate and elution of the GST fusion