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Description

Blots were washed with PBS + 0.1% Tween 20, three times 5 min each, and probed with secondary antibodies (Anti-rabbit IgG, HRP-linked Antibody, Cell Signaling #7074) at a concentration of 1:10,000 for 1 h at room temperature

The prescribed dose in milligrams is transferable between products

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Studies of e-cigarettes have shown the potentially harmful effects of flavourings in nicotine vape products, including toxicity to cells, oxidative stress and inflammation