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Cell lysates and tissue homogenates were denatured by incubation with Laemmli sample buffer (5.1% glycerol, 0.51 % SDS, 0.051% bromophenol blue, 30 mM DTT, 10.6 mM Tris-HCl, pH 6.9) for 5 min at 95 C

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however, human data remain limited and inconsistent

In conclusion, pro-inflammatory macrophages and their distinct modes of cell death mutually amplify each other, creating a positive feedback loop that exacerbates kidney injury