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Multi-photon and second harmonic generation microscopy The collagen gels were fixed with 4% paraformaldehyde for 20 minutes and washed in PBS with 0.1% saponin and 0.1% Tween 20 before being incubated with phalloidin conjugated with Alexa Fluor 594 for 1 h at room temp

It was submitted about 2 years ago

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The following exclusion criteria were applied: a history or presence of cholelithiasis, diabetes, tumours in the pituitary gland or hypothalamus, any serious allergy, positive serological test for hepatitis B or C and HIV, hypertension or other significant cardiovascular abnormality