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Description

Neuroscience 463 , 216226 (2021)

Regulatory R region of the CFTR chloride channel is a dynamic integrator of phospho-dependent intra- and intermolecular interactions

Materials and methods Generation of HEK293 cells stably expressing Grx1-roGFP1-iE ER HEK293 cells were transfected with Grx1-roGFP1-iE ER /pcDNA3.1 [13] using Metafectene PRO (Biontex) and stably expressing clones selected by addition of 1 mg/ml G418 (Sigma)