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Cells were fixed with 4% (v/v) formaldehyde in PBS for 15 min at RT, followed by staining with DAPI (1 g/ml)

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By treating the exact source of pain, this method increases the chance of lasting relief

A single 10mL or 30mL bacteriostatic water vial can be used to reconstitute multiple peptide vials, as long as: Sterile technique is maintained at every syringe draw A fresh, sterile needle is used each time The vial is used within 28 days of first opening The vial is stored refrigerated after opening One 10mL bacteriostatic water vial can easily reconstitute 3-5 research peptide vials depending on reconstitution volumes used