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ERYTHROCYTE ISOLATION Red blood cells were isolated from whole blood by density gradient centrifugation with FICOLL-Paque (GE Healthcare, 17-440-02)

There are many evidences that the changes of catalase activity as well as the mechanisms of its regulation are essential in the response to stress situations which catalyzes the dismutation of H2O2, forming O2 and H2O resulting good protection the cells from the toxic effects of hydrogen peroxide (Brioukhanov and Netrusor, 2004)

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