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Samples were washed twice with 50 mM TrisHCl (pH 7)10 mM EDTA for 20 min in order to remove copper ions from algal cell walls ( For the detection of GSH, ASC, and lipoperoxides, the alga (5 g) was cultivated in 30 ml of seawater containing 10 M of cadmium for 0, 1, 3, 5, and 7 days, in triplicate, and the culture medium was changed every 48 h

Jiang Y, Chen M, Nie H, Yuan Y

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