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NS-EM images of the SEC-purified complexes showed that the nanoparticles are ~35 nm in diameter and in the 2D class averages the I53-50 nanoparticle core and protruding E2E1 densities were clearly visible (Fig

We speculated that ORF3a-binding with VPS39 decomposes the HOPS complex, which leads to failure HOPSRab7 bundle formation, which is important for autophagosomelysosome fusion 46