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The most frequently observed adverse reactions were injection site pain in 7 patients (2.6 %), hypersensitivity (such as rash, fever, and itching) in 1 patient (0.4 %), injection site indurations in 1 patient (0.4 %), and gynaecomastia in 1 patient (0.4 %)

The supernatant was injected onto a 5-ml HisTrap HP column (Cytiva), and CENP-N/L complex was coeluted using 20 mM Tris, pH 7.5, 0.3 M NaCl, 5% glycerol, 5 mM BME, and 300 mM imidazole