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Melatonin restored the expression of genes involved in glycolysis (HK2, ENO1, PKM1, LDHA) and the TCA cycle (CS, IDH1, SUCLA2, FH, MDH1), reversed HO-induced metabolic disorders, increased the activity of mitochondrial oxidative phosphorylation complexes (II, III, and V), and increased the efficiency of ATP generation to safeguard the energy requirements of oocytes

In VRK2-WT cells, after monensin treatment for 6 h, most of SLC7A11 was redistributed to the Golgi, and then returned to the PM after monensin removal for 4 h

The participants with following evidence and symptoms were not included in the study

(3) Sperm midpiece/tail: Oxidative injury can inhibit metabolic enzymes and block ATP generation