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Cells were harvested at approximately 80% confluency by washing twice with PBS (Gibco, Invitrogen) and subsequently adding boiling GdmCl lysis buffer (6 M guanidine hydrochloride, 5 mM tris(2-carboxyethyl)phosphine, 10 mM chloroacetamide, 100 mM Tris pH 8.5) directly to the plate