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Cell lysates and NMR experiments in lysates U-251 MG human brain glioblastoma cells (CLS Cell Line Service, Eppelheim, Germany) were cultured in Dulbeccos Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum and 0.1% PenicillinStreptomycin (Biowest, Nuaill, France)

In this case, the binding was measured towards a recombinant truncated and soluble form of the receptor

Rakotoarisoa M, Angelova A (2018) Amphiphilic nanocarrier systems for curcumin delivery in neurodegenerative disorders

Semaglutide Semaglutide marketed as Ozempic for type 2 diabetes and Wegovy for chronic weight management is the most widely studied GLP-1 agonist in PCOS at this point