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In addition, mouse BMECs were also found to produce inflammatory responses upon LPS stimulation, specifically by directly producing inflammatory factors such as interleukin (IL)-1, IL-18, IL-6, and TNF-, and these factors are able to mediate the damaging effects of LPS on the BBB (Smyth et al., 2018

At the same time, these mediators drive heightened mitochondrial respiration in chondrocytes, generating excess reactive oxygen species (ROS) that damage mitochondria and promote chondrocyte apoptosis and autophagy (Vonk et al., 2018

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Furthermore, we identified the underlying molecular mechanisms: Wnt induction of IGF1 depends on -catenin and subsequent binding of Wnt transcription factor TCF4 to the IGF1 gene promoter