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The best fractions of protein peak were pooled, concentrated to 10 mg/mL with an Amicon Centrifugal filter (Millipore), flash-frozen in liquid nitrogen, and stored at 80 C for crystallization and activity assay

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Thiol Driving Roadmap Flowchart Phantasm Powder Its not just hops that have an incredible amount of bound thiol potential, but wine grapes (and other fruits) can have lots of bound potential, which is where most of this research started in the first place