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Spheroids were grown under standard tissue culture conditions in an incubator (37 C and 5% CO 2 )

In addition, GPX4 is also crucial in mediating the process of ferrozois, and the reduction of GPX4 activity has been found to promote the progression of ferrozoosis

Senolytic FOXO4-DRI can inhibit FOXO4 binding by disrupting the p53-FOXO4 interaction and selectively cause apoptosis via p53 nuclear exclusion in senescent cells and aging mouse models, which may be a promising approach for preventing senescence 11