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The Area under the curve (AUC) of each peak was normalized by an internal standard, and then the concentration of each lipid was calculated based on the previously reported NISTIR 8185 and LIPID MAPS references [40]

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According to NHS guidance (NHS Live Well: Lose Weight), a safe rate of weight loss is approximately 0.5 to 1 kg (1 to 2 lbs) per week , typically achieved through an energy deficit of around 600 kcal per day below total daily energy expenditure