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Samples were then mixed with an equal volume of metaphosphoric acid (10 g per 100 ml) and centrifuged at 580 g (2,500 r.p.m.) at 4 C for 5 min to precipitate protein

Ethics declarations Competing Interests The authors declare no competing interests

Although the overall eligibility range was 112 years, all participants who completed the dietary intervention and were included in the prepost metabolomic analyses were aged 4 years (range: 49 years