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In all three of these aforementioned clinical treatment studies performed by the authors, biomarkers of redox and methylation metabolism were measured

(2021) 118:e2009620118

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Addition of hydrophobic methyl group substitutions around the quinolinium scaffold (previously shown to negatively impact NNMT inhibitory activity) only slightly improved membrane permeability via passive transport as indicated by the low, but non-zero, permeability values for 1,8-diMQ and 1,2,4,8-tetraMQ (Table 4)