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Description

The resulting cDNA was subjected to quantitative real-time PCR using the Power SYBR Green PCR Master Mix (Applied Biosystems) according to the manufacturers instructions

Quantitative fluorescence intensities of CellROX Orange in the various groups are shown in Figure 3E to support the visual results and validate that the OH Py 2 N 2 molecule protects against ROS induced cell death by H 2 O 2 by decreasing the oxidative stress in the LECs