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This multistep, one-pot process proceeds via the intermediacy of the isothiouronium salt, which is hydrolyzed in a separate step: [24] [25] CH 3 CH 2 Br + SC(NH 2 ) 2 [CH 3 CH 2 SC(NH 2 ) 2 ]Br [CH 3 CH 2 SC(NH 2 ) 2 ]Br + NaOH CH 3 CH 2 SH + OC(NH 2 ) 2 + NaBr The thiourea route works well with primary halides, especially activated ones

However, there still exists a few limitations in this research

Nrf2 transcribes the genes encoding cytoprotective proteins such as catalase, glucose 6-phosphate dehydrogenase, superoxide dismutase, thioredoxin, glutathione peroxidase, and glutathione