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Subsequent to gel-purification, the fragments were digested with Spe I and Hind III, respectively

The ER membrane protein EI24 senses the elevation of H 2 O 2 levels in ER and facilitates the binding of RNA binding protein RNA transcription, translation, and transport factor (RTRAF) to the 3-untranslated region (UTR) of NOX4 messenger RNA (mRNA), inhibiting the translation of NOX4 and suppressing further H 2 O 2 production and redox damage [98], suggesting the self-regulation of ER ROS in maintaining oxidative homeostasis